PT Round 2025MWW02R1 Step 1 of 5 20% Instructions to Participants Please read carefully before starting analysis Quick Safety & Handling Only trained and authorized personnel should handle PT items. On receiving the PT item/sample, check that it is intact. If there is any issue, inform the PTP immediately. Record this in the sample receipt acknowledgement link shared by email and submit it within two days of receipt. Record date of receipt and store under the specified conditions until analysis. Initiate analysis within 48 hours of receipt. Treat PT items like routine samples and use standard methods unless otherwise instructed. Read the detailed instructions/information and follow them accordingly. Open PT containers only when you begin the analysis. Program Details Program Code: 2025MWW02R1 Category: Waste Water Measurand: Total Plate Count, and Faecal streptococci Result Submission: 15 January 2026 Storage: 2–8 °C until analysis Start Analysis Before:3 January 2026 Revival Instructions for Vials Vial 2025MWW02R1-1 Uncap the sample vial 2025MWW02R1-1 aseptically and rehydrate using 5ml sterile saline peptone water (0.1% peptone with 0.85% NaCl). Securely recap and allow 5 minutes rehydration at room temperature. Empty the rehydrate vial content to 500 ml of sterile distilled water, ensuring thorough homogenization of the sample. Treat this as your sample and carry out the analysis for the Faecal streptococci. Submit the results as MPN only. Vial 2025MWW02R1-2 Uncap the sample vial 2025MWW02R1-2 aseptically and rehydrate using 5ml sterile saline peptone water (0.1% peptone with 0.85% NaCl). Securely recap and allow 5 minutes rehydration at room temperature. Empty the rehydrate vial content to 100 ml of sterile distilled water, ensuring thorough homogenization of the sample. Treat this as your undilluted routine sample and carry out further dillutions from 10:100000 for Total Plate Coumt. Submit the results as cfu/ml only. Raw Data Submission Clear photographs of all plates (before counting, with readable labelling). Photographs of all biochemical / confirmation tests performed (if applicable). Accepted formats: PDF | Max size: 5 MB each. Ensure photos are clear, in focus, and labelled with sample code, date and dilution (if used). Note: Ensure images are in focus and labeled with sample ID, dilution, and date. Reporting of Results PT Round: 2025MWW02R1 Analytes to Report For this PT round 2025MWW02R1, you are required to report results for the following analytes: Total Plate Count Reporting Basis: cfu/ml IS 5401 (Part 1):2021 Faecal streptococci Reporting Basis: MPN/100ml APHA 9230 B 24th edition or IS 1622:1981 Online submission only: Do not download/print this page. Use the Next button to proceed to result entry. Key Instructions (Read before you begin) Do NOT download or print this page…results must be entered and submitted online only. Use the Next button at the bottom to go to the results entry section. Double-check all entries before submission — changes may not be accepted after Final submission. Retain all raw data and supporting records (e.g., plate photographs, biochemical results, calibration logs) as per ISO/IEC 17025. Upload clear evidence where required — unclear/missing records may lead to disqualification. Avoid collusion — responsibility lies entirely with the participant. Click Submit once only — unsolicited re-submissions may result in only the first entry being considered. If you cannot proceed, ensure all required fields are completed. You will see a confirmation message upon successful submission. Use the specified methods, or an equivalent validated method capable of required accuracy. Obtain prior written permission for deviations. Results in inappropriate formats (e.g., “less than 2 or 1.8;”, “More than 1600”, or zero where not applicable) may not be considered. Read and follow the PT Protocol carefully before starting analysis. Consent(Required) I have read, understood, and agree to the above instructions and conditions.Please click on the consent to proceed with the result submission. PT Result Submission Program Code: 2025MWW02R1 Last Date for Result Submission: 15th January 2026 Note: All fields are mandatory. Before starting the analysis, please ensure you have read the handling and analysis instructions carefully. Lab Code(Required)Please enter a number from 0 to 15.For lab code, refer to the sample forwarding letter sent along with the sampleName of Participating Laboratory/ Organization:(Required) Date of Result Reporting(Required) DD dot MM dot YYYY As filled in the registration formName(Required) Designation(Required) Phone(Required)Email(Required) Start Date of Analysis(Required) DD dot MM dot YYYY End Date of Analysis(Required) DD dot MM dot YYYY Section 1Sample Vial(Required)2025MWW02R1-1Please select the appropriate vial codeMeasurand/Parameter(Required)Faecal streptococciPlease select the appropriate measurandParticipating ? (Section 1)(Required)Select from the DropdownYes – I will submit results for this parameterNo – I am not participating in this parameterResults(Required)Please enter a number from 2 to 1600.Please don’t enter values in decimal points. Do not enter any symbols “greater than” or “less than”, etc. Values reported less that 2 or 1.8 wont be considered for final evaluation as per the standards, these values will be marked as RNC in the final report. Units of Measurement(Required)MPN/100mlMethod Used(Required)Any other equivalent methodAPHA 9230 B 24th editionIS 1622:1981Media Used(Required)Number of positive tubes(Required) Please report results in set format E.g.: “5-4-1”Incubation Conditions(Required)e.g. 37 Degrees for 24 hrsBiochemical Performed(Required)Any Confirmatory/Biochemical Tests Performed and their resultsAccredited as per ISO 17025 scope?(Required)YesNoApplicantSection 2Sample Vial(Required)2025MWW02R1-2Please select the appropriate vial codeMeasurand/Parameter(Required)Total Plate CountPlease select the appropriate measurandParticipating ? (Section 2)(Required)Select from the DropdownYes – I will submit results for this parameterNo – I am not participating in this parameterResults(Required)Please enter a number greater than or equal to 1.Please don’t enter values in decimal points. Do not enter any symbols “greater than” or “less than”, etc. Values reported less that 2 or 1.8 wont be considered for final evaluation as per the standards, these values will be marked as RNC in the final report. Units of Measurement(Required)cfu/mlMethod Used(Required)Any other equivalent methodIS 5401 (Part 1):2021Media Used(Required)Number of Dillutions(Required)Incubation Conditions(Required)e.g. 37 Degrees for 24 hrsBiochemical Test(Required)Any Confirmatory/Biochemical Tests Performed and their resultsAccredited as per ISO 17025 scope?(Required)YesNoApplicantUpload Raw Data(Required)Max. file size: 512 MB.Upload clear photographs of plates, positive tubes and biochemical tests performed for all measurand or parameter tested. Equipment Used(Required)Reference Material/s Used (Include Traceability))(Required)Were all the equipment used calibrated ?(Required)Choose from Drop DownYesNo(Choose Yes/No as per the applicability) Organizations’ DetailsAccredited I Mention the discipline(Required)BiologicalChemicalApplicantApplicant I Mention the discipline If NABL accredited mention TC No(Required) e.g. TC xxxxxTested By(Required) Designation(Required) Authorized By(Required) Designation(Required) Consent(Required) I confirm that all results are accurate and obtained as per scheme instructions.I hereby confirm that I am authorized to submit this form. I have thoroughly checked and updated all required fields to ensure accuracy. I fully understand that once this form is submitted, the results cannot be altered under any circumstances. Furthermore, I acknowledge and accept full responsibility for the accuracy and completeness of all information provided in this submission.
