PT Round 2025MF03R1 Step 1 of 5 20% Instructions to Participants Please read carefully before starting analysis Quick Safety & Handling Only trained and authorized personnel should handle PT items. Confirm sample integrity upon receipt; notify the PT Provider if damaged. Record date of receipt and store under the specified conditions until analysis. Initiate analysis within 48 hours of receipt. Treat PT items like routine samples and use standard methods unless otherwise instructed. Program Details Program Code: 2025MF03R1 Category: Fruits and Vegetable Products Matrix: Potato flakes Measurand: Salmonella, Escherichia coli, Shigella Listeria monocytogenes Result Submission: 30 Oct 2025 Storage: 2–8 °C until analysis Revival Instructions Vial 2025MF03R1-V1 Aseptically uncap the vial and rehydrate with 4 ml sterile saline peptone water (0.1% Peptone + 0.85% NaCl). Securely recap and allow 5 minutes rehydration at room temperature. Uncap container 2025MF03R1-1 and transfer 25 g of sample matrix to Salmonella enrichment broth and from 2025MF03R1-2 transfer 25 g to Shigella enrichment broth. Homogenize thoroughly. Transfer rehydrated vial 2ml each to Salmonella and Shigella enrichment broth ensuring proper homogenization. Perform routine analysis for: Salmonella and Shigella. Vial 2025MF03R1-V2 Aseptically uncap the vial and rehydrate with 4 ml sterile saline peptone water (0.1% Peptone + 0.85% NaCl). Securely recap and allow 5 minutes rehydration at room temperature. Uncap container 2025MF03R1-3 and transfer 25 g of sample matrix to the dilluting fluids for Echerichia coli and from 2025MF03R1-4 transfer 25g to the enrichment broth for L. monocytogenes. Homogenize thoroughly. Transfer rehydrated vial 2ml each to L. monocytogenes enrichment broth and E.coli diluting fluid ensuring proper homogenization. Perform routine analysis for: L. monocytogenes and Escherichia coli. Raw Data Submission Upload clear photographs of plates for all analytes tested. Upload photographs of biochemical tests performed. Accepted formats: JPG, PNG, PDF | Max size: 5 MB each. Note: Ensure images are in focus and labeled with sample ID, dilution, and date. Reporting of Results PT Round: 2025MF03R1 Analytes to Report For this PT round 2025MF03R1, you are required to report results for the following analytes: Salmonella Reporting Basis: Per 25 g IS 5887 (Part 3):1999 ISO 6579-1:2017 Shigella Reporting Basis: per 25 g IS 5887 (Part 7):1999 Listeria monocytogenes Reporting Basis: per 25 g IS 14988 (Part 1):2020 BAM chapter 10 Escherichia coli Reporting Basis: per g IS 5887 (Part 1):1976 Online submission only: Do not download/print this page. Use the Next button to proceed to result entry. Key Instructions (Read before you begin) Do NOT download or print this page — results must be entered and submitted online only. Use the Next button at the bottom to go to the results entry section. Double-check all entries before submission — changes may not be accepted after submission. Retain all raw data and supporting records (e.g., plate photographs, biochemical results, calibration logs) as per ISO/IEC 17025. Upload clear evidence where required — unclear/missing records may lead to disqualification. Avoid collusion — responsibility lies entirely with the participant. Click Submit once only — unsolicited re-submissions may result in only the first entry being considered. If you cannot proceed, ensure all required fields are completed. You will see a confirmation message upon successful submission. Use the specified methods, or an equivalent validated method capable of required accuracy. Obtain prior written permission for deviations. Results in inappropriate formats (e.g., “<”, “>”, or zero where not applicable) may not be considered. Read and follow the PT Protocol carefully before starting analysis. Consent(Required) I have read, understood, and agree to the above instructions and conditions.Please click on the consent to proceed with the result submission. PT Result Submission Program Code: 2025MF03R1 Last Date for Result Submission: 30th October 2025 Note: All fields are mandatory. Before starting the analysis, please ensure you have read the handling and analysis instructions carefully. Lab Code(Required)Please enter a number from 0 to 20.For lab code, refer to the sample forwarding letter sent along with the sampleName of Participating Laboratory/ Organization:(Required) Date of Result Reporting(Required) DD dot MM dot YYYY As filled in the registration formName(Required) Designation(Required) Phone(Required)Email(Required) Start Date of Analysis(Required) DD dot MM dot YYYY End Date of Analysis(Required) DD dot MM dot YYYY Section 1Sample Vial(Required)2025MF03R1-1Please select the appropriate vial codeMeasurand/Parameter(Required)SalmonellaE. coliL. monocytogenesS. aureusShigellaTotal coliformE.coliPlease select the appropriate measurandParticipating ?(Required)Yes – I will submit results for this parameterNo – I am not participating in this parameterResults(Required)DetectedNot DetectedEnter your results as “Present”, “Absent” only. Do not type numbers, “<”, “>” or “0”. Only required format is accepted.Units of Measurement(Required)Per gPer 25gMPN/100mlMethod Used(Required)Any other equivalent methodIS 5887 (Part 3):1999ISO 6579 (Part 1):2017IS 5887 (Part 7):1999IS 5887 (Part 1):1976IS 14988 (Part 1):2020BAM chapter 10Media Used(Required)Colony Characteristics(Required)Incubation Conditions(Required)e.g. 37 Degrees for 24 hrsBiochemical Performed(Required)Any Confirmatory/Biochemical Tests Performed and their resultsAccredited as per ISO 17025 scope?(Required)YesNoApplicantSection 2Sample Vial(Required)2025MF03R1-2Please select the appropriate vial codeMeasurand/Parameter(Required)SalmonellaE. coliL. monocytogenesS. aureusShigellaTotal coliformE.coliPlease select the appropriate measurandParticipating ?(Required)Yes – I will submit results for this parameterNo – I am not participating in this parameterResults(Required)AbsentPresentDetectedNot DetectedEnter your results as “Present”, “Absent” only. Do not type numbers, “<”, “>” or “0”. Only required format is accepted.Units of Measurement(Required)Per gPer 25gMPN/100mlMethod Used(Required)IS 5887 (Part 1):1976Any other equivalent methodIS 5887 (Part 7):1999ISO 6579 (Part 1):2017IS0 21528 (Part 2):2020BAM chapter 10IS 1622APHA 9221 24th EditionIS 14988 (Part 1): 2020ISO 11290 (Part 1): 2017IS 5887 (Part 3):1999Media Used(Required)Colony Charateristics(Required)Incubation Conditions(Required)e.g. 37 Degrees for 24 hrsBiochemical Test(Required)Any Confirmatory/Biochemical Tests Performed and their resultsAccredited as per ISO 17025 scope?(Required)YesNoApplicantSection 3Sample Vial(Required)2025MF03R1-3Please select the appropriate vial codeMeasurand/Parameter(Required)SalmonellaShigellaEscherichia coliListeria monocytogenesPlease select the appropriate measurandParticipating ?(Required)Yes – I will submit results for this parameterNo – I am not participating in this parameterResults(Required)AbsentDetectedAbsentNot DetectedEnter your results as “Present/Detected”, “Absent/Not Detected” only. Do not type numbers, “<”, “>” or “0”. Only required format is accepted.Units of Measurement(Required)Per gPer 25gMPN/100mlcfu/gMethod Used(Required)Any other equivalent methodIS 5887 (Part 3):1999ISO 6579 (Part 1):2017IS 5887 (Part 7):1999IS 5887 (Part 1):1976IS 14988 (Part 1):2020BAM chapter 10Media Used(Required)Colony Charateristics(Required)Incubation Conditions(Required)e.g. 37 Degrees for 24 hrsBiochemical Test(Required)Any Confirmatory/Biochemical Tests Performed and their resultsAccredited as per ISO 17025 scope?(Required)YesNoApplicantSection 4Sample Vial(Required)2025MF03R1-4Please select the appropriate vial codeMeasurand/Parameter(Required)Escherichia coliListeria monocytogenesShigellaSalmonellaPlease select the appropriate measurandParticipating ?(Required)Yes – I will submit results for this parameterNo – I am not participating in this parameterResultsDetectedNot detectedPresentAbsentEnter your results as “Present/Detected”, “Absent/Not Detected” only. Do not type numbers, “<”, “>” or “0”. Only required format is accepted.Units of Measurement(Required)Per gPer 25gMPN/100mlcfu/gMethod Used(Required)Any other equivalent methodIS 5887 (Part 6):2012IS 5887 (Part 1):1976BAM chapter 14IS 5402 (Part 1):2021BAM chapter 3IS 14988 (Part 1):2020BAM Chapter 10Media Used(Required)Colony Charateristics(Required)Incubation Conditions(Required)e.g. 37 Degrees for 24 hrsBiochemical Test(Required)Any Confirmatory/Biochemical Tests Performed and their resultsAccredited as per ISO 17025 scope?(Required)YesNoApplicantUpload Raw Data(Required)Max. file size: 512 MB.Upload clear photographs of plates, positive tubes and biochemical tests performed for all measurand or parameter tested. Equipment Used(Required)Reference Material/s Used (Include Traceability))(Required)Were all the equipment used calibrated ?(Required)Choose from Drop DownYesNo(Choose Yes/No as per the applicability) Organizations’ DetailsAccredited I Mention the discipline(Required)BiologicalChemicalApplicantApplicant I Mention the discipline If NABL accredited mention TC No(Required) e.g. TC xxxxxTested By Designation(Required) Authorized By Designation(Required) Consent(Required) I confirm that all results are accurate and obtained as per scheme instructions.I hereby confirm that I am authorized to submit this form. I have thoroughly checked and updated all required fields to ensure accuracy. I fully understand that once this form is submitted, the results cannot be altered under any circumstances. Furthermore, I acknowledge and accept full responsibility for the accuracy and completeness of all information provided in this submission.
