PT Round 2025MF15R1 Step 1 of 5 20% Instructions to Participants Only trained and authorized personnel should handle PT items. Confirm sample integrity upon receipt and notify the PT Provider if damaged. Record date of receipt and store under the specified conditions until analysis. Initiate analysis within 48 hours of receipt. Treat PT items like routine samples and use standard methods unless otherwise instructed. Program Details Program Code: 2025MF15R1 | Cereals, Pulses and Cereal Products Matrix: Cereal based Product Measurand: E. coli, Salmonella, S.aureus and L. monocytogenes Last Date of Result Submission: 15th September 2025 Storage Condition: 2–8°C until analysis Revival Instructions Vial 2025MF15R1-V1 Aseptically uncap the vial and rehydrate with 4 ml sterile saline peptone water (0.1% Peptone + 0.85% NaCl). Securely recap and allow 5 minutes rehydration at room temperature. Uncap container 2025MF15R1-1: Transfer 25 g of sample matrix to Salmonella enrichment broth. Transfer remaining 25 g to E. coli diluting fluid. Homogenize thoroughly. Transfer 2 ml rehydrated vial to Salmonella enrichment broth and remaining 2 ml to E. coli diluting fluid. Perform routine analysis for: Salmonella and E. coli. Vial 2025MF15R1-V2 Aseptically uncap the vial and rehydrate with 4 ml sterile saline peptone water (0.1% Peptone + 0.85% NaCl). Securely recap and allow 5 minutes rehydration at room temperature. Uncap container 2025MF15R1-2 and transfer 25 g of sample matrix to L. monocytogenes enrichment broth. Transfer remaining 25 g to S. aureus enrichment broth. Homogenize thoroughly. Transfer 2 ml rehydrated vial to L. monocytogenes enrichment broth and remaining 2 ml to S. aureus enrichment broth. Perform routine analysis for: L. monocytogenes and S. aureus. Raw Data Submission Requirements Upload clear photographs of plates for all analytes tested. Upload photographs of biochemical tests performed. Accepted formats: JPG, PNG, PDF | Max size: 5 MB each. Reporting of Results For this PT round 2025MF15R1, you are required to report results for the following analytes: E. coli — Per g | IS 5887 (Part 1):1976 Salmonella — Per 25 g | IS 5887 (Part 3):1999 / ISO 6579 (Part 1):2017 L. monocytogenes — Per 25 g | IS 14988 (Part 1):2020 / BAM Chapter 10 S. aureus — Per 25 g | IS 5887 (Part 2):1976 Key Instructions (Read before you begin) Do NOT download or print this page — results must be entered and submitted online only. Use the Next button at the bottom to go to the results entry section. Double-check all entries before submission — changes may not be accepted after submission. Retain all raw data and supporting records (e.g., photos of plates, biochemical test results, calibration logs) as per ISO/IEC 17025 requirements. Upload clear evidence where required — unclear or missing records may lead to disqualification. Do not engage in collusion — responsibility lies entirely with the participant. Click the Submit button once only — re-submissions without request may result in only the first entry being considered. If you cannot proceed, check that all required fields are completed. You will see a confirmation message once your results are successfully submitted. Use the specified methods, or an equivalent validated method capable of achieving required accuracy. Obtain prior written permission for deviations. Results in inappropriate formats (e.g., “less than”, “more than”, or zero where not applicable) may not be considered. Read and follow the PT Protocol carefully before starting analysis. Consent(Required) I have read, understood, and agree to the above instructions and conditions.Please click on the consent to proceed with the result submission. PT Result Submission Program Code: 2025MF15R1 Last Date for Result Submission: 15th September 2025 Note: All fields are mandatory. Before starting the analysis, please ensure you have read the handling and analysis instructions carefully. Lab Code(Required)Please enter a number from 0 to 17.For lab code, refer to the sample forwarding letter sent along with the sampleName of Participating Laboratory/ Organization:(Required) Date of Result Reporting(Required) DD dot MM dot YYYY As filled in the registration formName(Required) Designation(Required) Phone(Required)Email(Required) Start Date of Analysis(Required) DD dot MM dot YYYY End Date of Analysis(Required) DD dot MM dot YYYY Section 1Sample Vial(Required)2025MF15R1-V12025MF15R1-V2Please select the appropriate vial codeMeasurand/Parameter(Required)SalmonellaE. coliL. monocytogenesS. aureusShigellaTotal coliformE.coliPlease select the appropriate measurandParticipating ?(Required)Yes – I will submit results for this parameterNo – I am not participating in this parameterResults(Required)AbsentPresentDetectedNot DetectedEnter your results as “Present”, “Absent” only. Do not type numbers, “<”, “>” or “0”. Only required format is accepted.Units of Measurement(Required)Per gPer 25gMPN/100mlMethod Used(Required)IS 5887 (Part 1):1976Any other equivalent methodIS 5887 (Part 1):1999ISO 6579 (Part 1):2017IS0 21528 (Part 2):2020BAM chapter 10IS 1622APHA 9221 24th EditionIS 14988 (Part 1): 2020ISO 11290 (Part 1): 2017IS 5887 (Part 3):1999Media Used(Required)Colony Characteristics(Required)Incubation Conditions(Required)e.g. 37 Degrees for 24 hrsBiochemical Performed(Required)Any Confirmatory/Biochemical Tests Performed and their resultsAccredited as per ISO 17025 scope?(Required)YesNoApplicantSection 2Sample Vial(Required)2025MF15R1-V12025MF15R1-V2Please select the appropriate vial codeMeasurand/Parameter(Required)SalmonellaE. coliL. monocytogenesS. aureusShigellaTotal coliformE.coliPlease select the appropriate measurandParticipating ?(Required)Yes – I will submit results for this parameterNo – I am not participating in this parameterUnits of Measurement(Required)Per gPer 25gMPN/100mlResults(Required)AbsentPresentDetectedNot DetectedEnter your results as “Present”, “Absent” only. Do not type numbers, “<”, “>” or “0”. Only required format is accepted.Method Used(Required)IS 5887 (Part 1):1976Any other equivalent methodIS 5887 (Part 1):1999ISO 6579 (Part 1):2017IS0 21528 (Part 2):2020BAM chapter 10IS 1622APHA 9221 24th EditionIS 14988 (Part 1): 2020ISO 11290 (Part 1): 2017IS 5887 (Part 3):1999Media Used(Required)Colony Charateristics(Required)Incubation Conditions(Required)e.g. 37 Degrees for 24 hrsBiochemical Test(Required)Any Confirmatory/Biochemical Tests Performed and their resultsAccredited as per ISO 17025 scope?(Required)YesNoApplicantSection 3Sample Vial(Required)2025MF15R1-V22025MF15R1-V1Please select the appropriate vial codeMeasurand/Parameter(Required)SalmonellaE. coliL. monocytogenesS. aureusShigellaTotal coliformPlease select the appropriate measurandParticipating ?(Required)Yes – I will submit results for this parameterNo – I am not participating in this parameterResults(Required)AbsentPresentDetectedNot DetectedEnter your results as “Present”, “Absent” only. Do not type numbers, “<”, “>” or “0”. Only required format is accepted.Units of Measurement(Required)Per gPer 25gMPN/100mlMethod Used(Required)IS 5887 (Part 1):1976Any other equivalent methodIS 5887 (Part 1):1999ISO 6579 (Part 1):2017IS 5887 (Part 2):1976IS0 21528 (Part 2):2020BAM chapter 10IS 1622APHA 9221 24th EditionIS 14988 (Part 1): 2020ISO 11290 (Part 1): 2017IS 5887 (Part 3):1999Media Used(Required)Colony Charateristics(Required)Incubation Conditions(Required)e.g. 37 Degrees for 24 hrsBiochemical Test(Required)Any Confirmatory/Biochemical Tests Performed and their resultsAccredited as per ISO 17025 scope?(Required)YesNoApplicantSection 4Measurand/Parameter(Required)SalmonellaE. coliL. monocytogenesS. aureusShigellaTotal coliformPlease select the appropriate measurandSample Vial(Required)2025MF15R1-V22025MF15R1-V1Please select the appropriate vial codeParticipating ?(Required)Yes – I will submit results for this parameterNo – I am not participating in this parameterResults(Required)AbsentPresentDetectedNot DetectedEnter your results as “Present”, “Absent” only. Do not type numbers, “<”, “>” or “0”. Only required format is accepted.Units of Measurement(Required)Per gPer 25gMPN/100mlMethod Used(Required)IS 5887 (Part 1):1976Any other equivalent methodIS 5887 (Part 1):1999ISO 6579 (Part 1):2017IS 5887 (Part 2):1976IS0 21528 (Part 2):2020BAM chapter 10IS 1622APHA 9221 24th EditionIS 14988 (Part 1): 2020ISO 11290 (Part 1): 2017IS 5887 (Part 3):1999Media Used(Required)Colony Charateristics(Required)Incubation Conditions(Required)e.g. 37 Degrees for 24 hrsBiochemical Test(Required)Any Confirmatory/Biochemical Tests Performed and their resultsAccredited as per ISO 17025 scope?(Required)YesNoApplicantUpload Raw Data(Required)Max. file size: 512 MB.Upload clear photographs of plates, positive tubes and biochemical tests performed for all measurand or parameter tested. Equipment Used(Required)Reference Material/s Used (Include Traceability))(Required)Were all the equipment used calibrated ?(Required)Choose from Drop DownYesNo(Choose Yes/No as per the applicability) Organizations’ DetailsAccredited I Mention the discipline(Required)BiologicalChemicalApplicantApplicant I Mention the discipline If NABL accredited mention TC No(Required) e.g. TC xxxxxTested By Designation(Required) Authorized By Designation(Required) Consent(Required) I confirm that all results are accurate and obtained as per scheme instructions.I hereby confirm that I am authorized to submit this form. I have thoroughly checked and updated all required fields to ensure accuracy. I fully understand that once this form is submitted, the results cannot be altered under any circumstances. Furthermore, I acknowledge and accept full responsibility for the accuracy and completeness of all information provided in this submission.
