Instructions and Result Submission: 2025MF05R2

PT Round 2025MF05R2

Step 1 of 5

Instructions to Participants

Please read carefully before starting analysis

Quick Safety & Handling

Only trained and authorized personnel should handle PT items.
Confirm sample integrity upon receipt; notify the PT Provider if damaged.
Record date of receipt and store under the specified conditions until analysis.
Initiate analysis within 48 hours of receipt.
Treat PT items like routine samples and use standard methods unless otherwise instructed.

Program Details

Program Code: 2025MF05R2 Category: Herbs, Spices & Condiments Matrix: Coriander Seeds Measurand: Salmonella, Bacillus cereus, Total Plate Count Result Submission: 30 Sep 2025 Storage: 2–8 °C until analysis

Revival Instructions

Vial 2025MF05R2-V1

Aseptically uncap the vial and rehydrate with 4 ml sterile saline peptone water (0.1% Peptone + 0.85% NaCl).
Securely recap and allow 5 minutes rehydration at room temperature.
Uncap container 2025MF05R2-1 and transfer 25 g of sample matrix to Salmonella enrichment broth.
Homogenize thoroughly.
Perform routine analysis for: Salmonella.

Vial 2025MF05R2-V2

Aseptically uncap the vial and rehydrate with 4 ml sterile saline peptone water (0.1% Peptone + 0.85% NaCl).
Securely recap and allow 5 minutes rehydration at room temperature.
Uncap container 2025MF05R2-1 and transfer 25 g of sample matrix to Salmonella enrichment broth.
Homogenize thoroughly.
Perform routine analysis for: Salmonella.

Vial 2025MF05R2-V3

Aseptically uncap the vial and rehydrate with 5 ml sterile saline peptone water (0.1% Peptone + 0.85% NaCl).
Securely recap and allow 5 minutes rehydration at room temperature.
Uncap container 2025MF05R2-2:
- Transfer 10 g of sample matrix to a sterile bag/bottle/flask and add 85 ml of diluent.
- Empty vial 2025MF05R2-V3 into the prepared diluent.
Homogenize thoroughly.
Perform serial dilution to 10⁻⁵ (i.e., 1:100,000) and proceed with routine analysis for: Total Plate Count and Bacillus cereus.

Raw Data Submission

Upload clear photographs of plates for all analytes tested.
Upload photographs of biochemical tests performed.
Accepted formats: JPG, PNG, PDF | Max size: 5 MB each.

Note: Ensure images are in focus and labeled with sample ID, dilution, and date.

Reporting of Results

PT Round: 2025MF05R2

Analytes to Report

For this PT round 2025MF05R2, you are required to report results for the following analytes:

Salmonella

Reporting Basis: Per 25 g

IS 5887 (Part 3):1999 ISO 6579-1:2017

Bacillus cereus

Reporting Basis: cfu per g

IS 5887 (Part 6):2012 BAM Chapter 14

Total Plate Count

Reporting Basis: cfu per g

IS 5402 (Part 1):2021 BAM Chapter 3

Online submission only: Do not download/print this page. Use the Next button to proceed to result entry.

Key Instructions (Read before you begin)

Do NOT download or print this page — results must be entered and submitted online only.
Use the Next button at the bottom to go to the results entry section.
Double-check all entries before submission — changes may not be accepted after submission.
Retain all raw data and supporting records (e.g., plate photographs, biochemical results, calibration logs) as per ISO/IEC 17025.
Upload clear evidence where required — unclear/missing records may lead to disqualification.
Avoid collusion — responsibility lies entirely with the participant.
Click Submit once only — unsolicited re-submissions may result in only the first entry being considered.
If you cannot proceed, ensure all required fields are completed.
You will see a confirmation message upon successful submission.
Use the specified methods, or an equivalent validated method capable of required accuracy. Obtain prior written permission for deviations.
Results in inappropriate formats (e.g., “<”, “>”, or zero where not applicable) may not be considered.
Read and follow the PT Protocol carefully before starting analysis.

PT Result Submission

Program Code: 2025MF05R2

Last Date for Result Submission: 30th September 2025

Note: All fields are mandatory.

Before starting the analysis, please ensure you have read the handling and analysis instructions carefully.

Lab Code(Required)

Please enter a number from 0 to 17.

For lab code, refer to the sample forwarding letter sent along with the sample

Name of Participating Laboratory/ Organization:(Required)

Date of Result Reporting(Required)

As filled in the registration form

Name(Required)

Designation(Required)

Phone(Required)

Email(Required)

Start Date of Analysis(Required)

End Date of Analysis(Required)

Section 1

Sample Vial(Required)

Please select the appropriate vial code

Measurand/Parameter(Required)

Please select the appropriate measurand

Participating ?(Required)

Results(Required)

Enter your results as “Present”, “Absent” only. Do not type numbers, “<”, “>” or “0”. Only required format is accepted.

Units of Measurement(Required)

Method Used(Required)

Media Used(Required)

Colony Characteristics(Required)

Incubation Conditions(Required)

e.g. 37 Degrees for 24 hrs

Biochemical Performed(Required)

Any Confirmatory/Biochemical Tests Performed and their results

Accredited as per ISO 17025 scope?(Required)

Section 2

Sample Vial(Required)

Please select the appropriate vial code

Measurand/Parameter(Required)

Please select the appropriate measurand

Participating ?(Required)

Results(Required)

Enter your results as “Present”, “Absent” only. Do not type numbers, “<”, “>” or “0”. Only required format is accepted.

Units of Measurement(Required)

Method Used(Required)

Media Used(Required)

Colony Charateristics(Required)

Incubation Conditions(Required)

e.g. 37 Degrees for 24 hrs

Biochemical Test(Required)

Any Confirmatory/Biochemical Tests Performed and their results

Accredited as per ISO 17025 scope?(Required)

Section 3

Sample Vial(Required)

Please select the appropriate vial code

Measurand/Parameter(Required)

Please select the appropriate measurand

Participating ?(Required)

Results

Please do not add “0”, “<" or ">” values in the field. The results should be the whole numbers. Decimals will not be accepted. Results that deviated this will not be considered for evaluation.

Units of Measurement(Required)

Method Used(Required)

Media Used(Required)

Colony Charateristics(Required)

Incubation Conditions(Required)

e.g. 37 Degrees for 24 hrs

Biochemical Test(Required)

Any Confirmatory/Biochemical Tests Performed and their results

Accredited as per ISO 17025 scope?(Required)

Section 4

Sample Vial(Required)

Please select the appropriate vial code

Measurand/Parameter(Required)

Please select the appropriate measurand

Participating ?(Required)

Results

Units of Measurement(Required)

Method Used(Required)

Media Used(Required)

Colony Charateristics(Required)

Incubation Conditions(Required)

e.g. 37 Degrees for 24 hrs

Biochemical Test(Required)

Any Confirmatory/Biochemical Tests Performed and their results

Accredited as per ISO 17025 scope?(Required)

Upload Raw Data(Required)

Max. file size: 512 MB.

Upload clear photographs of plates, positive tubes and biochemical tests performed for all measurand or parameter tested.