PT Round 2026MN02R1 Step 1 of 5 20% Instructions to Participants Please read carefully before starting analysis Quick Safety & Handling Only trained and authorized personnel should handle PT items. Confirm sample integrity upon receipt; notify the PT Provider if damaged. Record date of receipt and store under the specified conditions until analysis. Initiate analysis within 48 hours of receipt. Treat PT items like routine samples and use standard methods unless otherwise instructed. Program Details Program Code: 2026MN02R1 Category:Probiotics Measurand: Salmonella, Listeria monocytogenes and Lactic Acid Bacteria Result Submission: 05 October 2026 Storage: 2–8 °C until analysis Revival Instructions Vial 2026MN02R1-V1 Uncap the PT item vial 2026MN02R1-V1 aseptically and rehydrate using 4ml sterile saline peptone water (0.1% Peptone with 0.85%NaCl). Recap securely and allow 5 minutes at room temperature for rehydration. Aseptically transfer 25g of PT item matrix from the PT item container 2026MN02R1 to the enrichment broth for Salmonella and the remaining 25g to the enrichment broth for Listeria monocytogenes. Transfer 2ml each of the rehydrated content from vial 2026MN02R1-V1 to the separately prepared the enrichment broth for Salmonella & Listeria monocytogenes ensuring thorough homogenization. Proceed with the routine analysis. Vial 2026MN02R1-V2 Uncap the PT item vial 2026MN02R1-V2 aseptically and rehydrate using 4ml sterile saline peptone water (0.1% Peptone with 0.85%NaCl). Aseptically transfer 10 g of the sample matrix from 2026MN02R1 into a sterile bag, bottle, or flask. Add 85 ml of the diluent as per standard. Empty the content of vial 2026MN02R1-V2 into the prepared 85 ml of diluent, ensuring thorough homogenization. Allow the mixture to stand for 5 minutes. Perform serial dilutions from 10¹ to 10⁶ and proceed with routine analysis for Enumeration of lactic acid bacteria as per standard procedures. Raw Data Submission Upload clear photographs of plates for all analytes tested. Upload photographs of biochemical tests performed. Accepted formats: PDF | Max size: 5 MB each. Note: Ensure images are in focus and labeled with sample ID, dilution, and date. Reporting of Results PT Round: 2026MN02R1 Analytes to Report For this PT round 2026MN02R1, you are required to report results for the following analytes: Salmonella Reporting Basis: Per 25g IS 5887 (Part 3):1999 ISO 6579 (Part 1): 2017 Lactic Acid Bacteria Reporting Basis: cfu/g ISO 15214:1998 RA 2021 Listeria monocytogenes Reporting Basis: Per 25g IS 14988 (Part 1):2020 BAM chapter 10 Online submission only: Do not download/print this page. Use the Next button to proceed to result entry. Key Instructions (Read before you begin) Do NOT download or print this page — results must be entered and submitted online only. Use the Next button at the bottom to go to the results entry section. Double-check all entries before submission — changes may not be accepted after submission. Retain all raw data and supporting records (e.g., plate photographs, biochemical results, calibration logs) as per ISO/IEC 17025. Upload clear evidence where required — unclear/missing records may lead to disqualification. Avoid collusion — responsibility lies entirely with the participant. Click Submit once only — unsolicited re-submissions may result in only the first entry being considered. If you cannot proceed, ensure all required fields are completed. You will see a confirmation message upon successful submission. Use the specified methods, or an equivalent validated method capable of required accuracy. Obtain prior written permission for deviations. Results in inappropriate formats (e.g., “<”, “>”, or zero where not applicable) may not be considered. Read and follow the PT Protocol carefully before starting analysis. Consent(Required) I have read, understood, and agree to the above instructions and conditions.Please click on the consent to proceed with the result submission. PT Result Submission Program Code: 2026MN02R1 Last Date for Result Submission: 05th October 2026 Note: All fields are mandatory. Before starting the analysis, please ensure you have read the handling and analysis instructions carefully. Name of Participating Laboratory/ Organization:(Required) Date of Result Reporting(Required) DD dot MM dot YYYY As filled in the registration formLab Code(Required)Please enter a number from 0 to 25.Sample Code(Required)Please enter a number from 0 to 25.Name(Required) Designation(Required) Phone(Required)Email(Required) Start Date of Analysis(Required) DD dot MM dot YYYY End Date of Analysis(Required) DD dot MM dot YYYY Section 1Sample Vial(Required)2026MN02R1-V1Please select the appropriate vial codeMeasurand/Parameter(Required)SalmonellaPlease select the appropriate measurandParticipating ?(Required)Select from the DropdownYes – I will submit results for this parameterNo – I am not participating in this parameterResults(Required)DetectedNot DetectedReport as “Detected/Not Detected” onlyUnits of Measurement(Required)Per 25gMethod Used(Required)Any other equivalent methodIS 5887 (Part 3):1999ISO 6579 (Part 1): 2017Media Used(Required)Incubation Conditions(Required)e.g. 37 Degrees for 24 hrsBiochemical Performed(Required)Any Confirmatory/Biochemical Tests Performed and their resultsAccredited as per ISO 17025 scope?(Required)YesNoApplicantSection 2Sample Vial(Required)2026MN02R1-V1Please select the appropriate vial codeMeasurand/Parameter(Required)Listeria monocytogenesPlease select the appropriate measurandParticipating ?(Required)Select from the DropdownYes – I will submit results for this parameterNo – I am not participating in this parameterResults(Required)DetectedNot DetectedReport as “Detected/Not Detected” onlyUnits of Measurement(Required)Per 25gMethod Used(Required)Any other equivalent methodIS 14988 (Part 1):2020ISO 11290 (Part 1):2017BAM chapter 10Media Used(Required)Incubation Conditions(Required)e.g. 37 Degrees for 24 hrsBiochemical Performed(Required)Any Confirmatory/Biochemical Tests Performed and their resultsAccredited as per ISO 17025 scope?(Required)YesNoApplicantSection 3Sample Vial(Required)2026MN02R1-V2Please select the appropriate vial codeMeasurand/Parameter(Required)Lactic Acid BacteriaPlease select the appropriate measurandParticipating ?(Required)Select from the DropdownYes – I will submit results for this parameterNo – I am not participating in this parameterResults(Required) Do not enter values in decimal points or symbols “greater than” or “less than”, etc. Values reported less that 2 or 1.8 wont be considered for final evaluation as per the standards, these values will be marked as RNC in the final report. Units of Measurement(Required)cfu/gMethod Used(Required)Any other equivalent methodISO 15214:1998 RA 2021Media Used(Required)Incubation Conditions(Required)e.g. 37 Degrees for 24 hrsBiochemical Performed(Required)Any Confirmatory/Biochemical Tests Performed and their resultsAccredited as per ISO 17025 scope?(Required)YesNoApplicantUpload Raw Data(Required)Max. file size: 512 MB.Upload clear photographs of plates, positive tubes and biochemical tests performed for all measurand or parameter tested. Equipment Used(Required)Reference Material/s Used (Include Traceability))(Required)Were all the equipment used calibrated ?(Required)Choose from Drop DownYesNo(Choose Yes/No as per the applicability) Organizations’ DetailsAccredited I Mention the discipline(Required)BiologicalChemicalApplicantApplicant I Mention the discipline If NABL accredited mention TC No(Required) e.g. TC xxxxxTested By(Required) Designation(Required) Authorized By(Required) Designation(Required) Consent(Required) I confirm that all results are accurate and obtained as per scheme instructions.I hereby confirm that I am authorized to submit this form. I have thoroughly checked and updated all required fields to ensure accuracy. I fully understand that once this form is submitted, the results cannot be altered under any circumstances. Furthermore, I acknowledge and accept full responsibility for the accuracy and completeness of all information provided in this submission.
