PT Round 2026MM01R1 Step 1 of 6 16% Instructions to Participants EL-F-01-7.3-06 Please read carefully before starting analysis Quick Safety & Handling Only trained and authorized personnel should handle PT items. Confirm sample integrity upon receipt; notify the PT Provider if damaged. Record date of receipt and store under the specified conditions until analysis. Initiate analysis within 48 hours of receipt. Treat PT items like routine samples and use standard methods unless otherwise instructed. Program Details Program Code: 2026MM01R1 Category:Marine /Aqua culture Food Products Matrix: Shrimps Measurand: Salmonella, Total Plate Count and Escherichia coli Result Submission: 12 August 2026 Storage: 2–8 °C until analysis Revival Instructions Vial 2026MM01R1-V1 Uncap the PT item vial 2026MM01R1-V1 aseptically and rehydrate using 4ml sterile saline peptone water (0.1% Peptone with 0.85%NaCl). Recap securely and allow 5 minutes at room temperature for rehydration. Aseptically transfer 25g of PT item matrix from the PT item container 2026MM01R1-1 to the enrichment broth for Salmonella. Transfer entire of the rehydrated content from vial 2026MM01R1-V1 to the enrichment broth for Salmonella ensuring thorough homogenization. Proceed with the routine analysis. Vial 2026MM01R1-V2 Uncap the PT item vial 2026MM01R1-V2 aseptically and rehydrate using 4ml sterile saline peptone water (0.1% Peptone with 0.85%NaCl). Recap securely and allow 5 minutes at room temperature for rehydration. Aseptically transfer 25g of PT item matrix from the PT item container 2026MM01R1-2 to the enrichment broth for Salmonella. Transfer entire of the rehydrated content from vial 2026MM01R1-V2 to the enrichment broth for Salmonella ensuring thorough homogenization. Proceed with the routine analysis. Vial 2026MM01R1-V3 Uncap the PT item vial 2026MM01R1-V3 aseptically and rehydrate using 5ml sterile saline peptone water (0.1% peptone with 0.85% NaCl). Recap securely & allow 5 minutes at room temperature for rehydration. Aseptically transfer 10 g of the PT item matrix from 2026MM01R1-3 to the sterile bag/bottle/flask & add 85 ml of diluent to each bag/bottle/flask. Empty the rehydrated vial to the diluent (prepared in step 3), ensuring thorough homogenization of the PT item. Incubate the prepared sample at 37°C for 2 hours and perform serial dilution: (101 – 105) & proceed with your routine analysis for Escherichia coli & Total plate count. Raw Data Submission Upload clear photographs of plates for all analytes tested. Upload photographs of biochemical tests performed. Accepted formats: JPG, PNG, PDF | Max size: 5 MB each. Note: Ensure images are in focus and labeled with sample ID, dilution, and date. Reporting of Results PT Round: 2026MM01R1 Analytes to Report For this PT round 2026MM01R1, you are required to report results for the following analytes: Total Plate Count Reporting Basis: cfu/ g IS 5402 (Part 1):2021, BAM chapter 3 Escherichia coli Reporting Basis: cfu/ g ISO 16649 (Part 1):2018 Salmonella Reporting Basis: per 25g IS 5887 (Part 3):1999, ISO 6579 (Part 1):2017 Online submission only: Do not download/print this page. Use the Next button to proceed to result entry. Key Instructions (Read before you begin) Do NOT download or print this page — results must be entered and submitted online only. Use the Next button at the bottom to go to the results entry section. Double-check all entries before submission — changes may not be accepted after submission. Retain all raw data and supporting records (e.g., plate photographs, biochemical results, calibration logs) as per ISO/IEC 17025. Upload clear evidence where required — unclear/missing records may lead to disqualification. Avoid collusion — responsibility lies entirely with the participant. Click Submit once only — unsolicited re-submissions may result in only the first entry being considered. If you cannot proceed, ensure all required fields are completed. You will see a confirmation message upon successful submission. Use the specified methods, or an equivalent validated method capable of required accuracy. Obtain prior written permission for deviations. Results in inappropriate formats (e.g., “<”, “>”, or zero where not applicable) may not be considered. Read and follow the PT Protocol carefully before starting analysis. Consent(Required) I have read, understood, and agree to the above instructions and conditions.Please click on the consent to proceed with the result submission. PT Result Submission EL-F-01-7.3-05 Program Code: 2026MM01R1 Last Date for Result Submission: 12th August 2026 Note: All fields are mandatory. Before starting the analysis, please ensure you have read the handling and analysis instructions carefully. Lab Code(Required)Please enter a number from 0 to 20.For lab code, refer to the sample forwarding letter sent along with the sampleName of Participating Laboratory/ Organization:(Required) As filled in the registration formDate of Result Reporting(Required) DD dot MM dot YYYY Sample Code(Required)For sample code, refer to the sample forwarding letter sent along with the sampleName(Required) Designation(Required) Phone(Required)Email(Required) Start Date of Analysis(Required) DD dot MM dot YYYY End Date of Analysis(Required) DD dot MM dot YYYY Section 1Sample Vial(Required)2026MM01R1-V1Please select the appropriate vial codeMeasurand/Parameter(Required)SalmonellaPlease select the appropriate measurandParticipating ?(Required)Yes – I will submit results for this parameterNo – I am not participating in this parameterResults(Required)DetectedNot DetectedUnits of Measurement(Required)per gper 25gMethod Used(Required)Any other equivalent methodIS 5887 (Part 3):1999ISO 6579 (Part 1):2017Media Used(Required)Colony characteristics(Required)Incubation Conditions(Required)e.g. 37 Degrees for 24 hrsBiochemical Performed(Required)Any Confirmatory/Biochemical Tests Performed and their resultsAccredited as per ISO 17025 scope?(Required)YesNoApplicantSection 2Sample Vial(Required)2026MM01R1-V2Please select the appropriate vial codeMeasurand/Parameter(Required)SalmonellaPlease select the appropriate measurandParticipating ?(Required)Yes – I will submit results for this parameterNo – I am not participating in this parameterResults(Required)DetectedNot DetectedUnits of Measurement(Required)per gper 25gMethod Used(Required)Any other equivalent methodIS 5887 (Part 3):1999ISO 6579 (Part 1):2017Media Used(Required)Colony characteristics(Required)Incubation Conditions(Required)e.g. 37 Degrees for 24 hrsBiochemical Test(Required)Any Confirmatory/Biochemical Tests Performed and their resultsAccredited as per ISO 17025 scope?(Required)YesNoApplicantSection 3Sample Vial(Required)2026MM01R1-V3Please select the appropriate vial codeMeasurand/Parameter(Required)Escherichia coliPlease select the appropriate measurandParticipating ?(Required)Yes – I will submit results for this parameterNo – I am not participating in this parameterResults(Required)Please enter a number greater than or equal to 0.Do not enter values in decimal points or symbols “greater than” or “less than”, etc. Values reported less that 2 or 1.8 wont be considered for final evaluation as per the standards, these values will be marked as RNC in the final report. Units of Measurement(Required)cfu/gMethod Used(Required)Any other equivalent methodISO 16649 (Part 1):2018Media Used(Required)Colony Charateristics(Required)Incubation Conditions(Required)e.g. 37 Degrees for 24 hrsBiochemical Test(Required)Any Confirmatory/Biochemical Tests Performed and their resultsAccredited as per ISO 17025 scope?(Required)YesNoApplicantSection 4Sample Vial(Required)2026MM01R1-V3Please select the appropriate vial codeMeasurand/Parameter(Required)Total Plate CountPlease select the appropriate measurandParticipating ?(Required)Yes – I will submit results for this parameterNo – I am not participating in this parameterResults(Required)Please enter a number greater than or equal to 0.Do not enter values in decimal points or symbols “greater than” or “less than”, etc. Values reported less that 2 or 1.8 wont be considered for final evaluation as per the standards, these values will be marked as RNC in the final report. Units of Measurement(Required)cfu/gMethod Used(Required)Any other equivalent methodIS 5402 (Part 1):2021BAM chapter 3Media Used(Required)Colony Charateristics(Required)Incubation Conditions(Required)e.g. 37 Degrees for 24 hrsBiochemical Test(Required)Any Confirmatory/Biochemical Tests Performed and their resultsAccredited as per ISO 17025 scope?(Required)YesNoApplicant Upload Raw Data(Required)Max. file size: 512 MB.Upload clear photographs of plates, positive tubes and biochemical tests performed for all measurand or parameter tested. Equipment Used(Required)Reference Material/s Used (Include Traceability))(Required)Were all the equipment used calibrated ?(Required)Choose from Drop DownYesNo(Choose Yes/No as per the applicability) Organizations’ DetailsAccredited I Mention the discipline(Required)BiologicalChemicalApplicantApplicant I Mention the discipline If NABL accredited mention TC No(Required) e.g. TC xxxxxTested By(Required) Designation(Required) Authorized By(Required) Designation(Required) Consent(Required) I confirm that all results are accurate and obtained as per scheme instructions.I hereby confirm that I am authorized to submit this form. I have thoroughly checked and updated all required fields to ensure accuracy. I fully understand that once this form is submitted, the results cannot be altered under any circumstances. Furthermore, I acknowledge and accept full responsibility for the accuracy and completeness of all information provided in this submission.
