2026MF44R1 Instruction sheet and Result submission

PT Round 2026MF44R1

Step 1 of 6

Please read carefully before starting analysis

Quick Safety & Handling

Only trained and authorized personnel should handle PT items.
Confirm sample integrity upon receipt; notify the PT Provider if damaged.
Record date of receipt and store under the specified conditions until analysis.
Initiate analysis within 48 hours of receipt.
Treat PT items like routine samples and use standard methods unless otherwise instructed.

Program Details

Program Code: 2026MF44R1 Category: Jams, Juices and Concentrates Matrix: Jams Measurand: Salmonella, Staphylococcus aureus, Listeria monocytogenes,
Total Plate Count and Yeast and Mould Result Submission: 05 August 2026 Storage: 2–8 °C until analysis

Revival Instructions

Vial 2026MF44R1-V1

Uncap the PT item vial 2026MF44R1-V1 aseptically and rehydrate using 4ml sterile saline peptone water (0.1% Peptone with 0.85%NaCl).
Recap securely and allow 5 minutes at room temperature for rehydration.
Aseptically transfer 25g of PT item matrix from the PT item container 2026MF44R1 to the enrichment broth for Salmonella and the remaining 25g to the enrichment broth for Listeria monocytogenes.
Transfer 2ml each of the rehydrated content from vial 2026MF44R1-V1 to the separately prepared the enrichment broth for Salmonella & Listeria monocytogenes ensuring thorough homogenization.
Proceed with the routine analysis.

Vial 2026MFF44R1-V2

Uncap the PT item vial 2026MF44R1-V2 aseptically and rehydrate using 4ml sterile saline peptone water (0.1% peptone with 0.85% NaCl).
Recap securely & allow 5 minutes at room temperature for rehydration.
Aseptically transfer the remaining 25 g of the PT item matrix from the container 2026MF44R1 to enrichment broth for Staphylococcus aureus by ensuring proper homogenization.
Empty the vial 2026MF44R1-V2 content to the diluent prepared in step 3 and ensure proper homogenization.
Proceed with your routine analysis.

Vial 2026MFF44R1-V3

Uncap the PT item vial 2026MF44R1-V3 aseptically and rehydrate using 5ml sterile saline peptone water (0.1% peptone with 0.85% NaCl).
Recap securely & allow 5 minutes at room temperature for rehydration.
Aseptically transfer 10 g of the PT item matrix from 2026MF44R1 to the sterile bag/bottle/flask & add 85 ml of diluent to each bag/bottle/flask.
Empty the rehydrated vial to the diluent (prepared in step 3), ensuring thorough homogenization of the PT item.
Perform serial dilution: 10¹-10⁵ & proceed with your routine analysis for Total plate count.

Vial 2026MFF44R1-V4

Uncap the PT item vial 2026MF44R1-V4 aseptically and rehydrate using 5ml sterile saline peptone water (0.1% peptone with 0.85% NaCl).
Recap securely & allow 5 minutes at room temperature for rehydration.
Aseptically transfer 10 g of the PT item matrix from 2026MF44R1 to the sterile bag/bottle/flask & add 85 ml of diluent to each bag/bottle/flask.
Empty the rehydrated vial to the diluent (prepared in step 3), ensuring thorough homogenization of the PT item.
Perform serial dilution: 10¹-10⁵ & proceed with your routine analysis for Yeast & Mould.

Raw Data Submission

Upload clear photographs of plates for all analytes tested.
Upload photographs of biochemical tests performed.
Accepted formats: JPG, PNG, PDF | Max size: 5 MB each.

Note: Ensure images are in focus and labeled with sample ID, dilution, and date.

Reporting of Results

PT Round: 2026MF44R1

Analytes to Report

For this PT round 2026MF44R1, you are required to report results for the following analytes:

Salmonella

Reporting Basis: per 25 g

IS 5887 (Part 3):1999
ISO 6579 (Part 1):2017

Listeria monocytogenes

Reporting Basis: per 25 g

IS 14988 (Part 1):2020
BAM chapter 10

Staphylococcus aureus

Reporting Basis: per 25 g

IS 5887 (Part 2):1976

Total Plate Count

Reporting Basis: cfu/ g

IS 5402 (Part 1):2021
BAM chapter 3

Yeast & Mould

Reporting Basis: cfu/g

IS 5403:1999
BAM chapter 18

Online submission only: Do not download/print this page. Use the Next button to proceed to result entry.

Key Instructions (Read before you begin)

Do NOT download or print this page — results must be entered and submitted online only.
Use the Next button at the bottom to go to the results entry section.
Double-check all entries before submission — changes may not be accepted after submission.
Retain all raw data and supporting records (e.g., plate photographs, biochemical results, calibration logs) as per ISO/IEC 17025.
Upload clear evidence where required — unclear/missing records may lead to disqualification.
Avoid collusion — responsibility lies entirely with the participant.
Click Submit once only — unsolicited re-submissions may result in only the first entry being considered.
If you cannot proceed, ensure all required fields are completed.
You will see a confirmation message upon successful submission.
Use the specified methods, or an equivalent validated method capable of required accuracy. Obtain prior written permission for deviations.
Results in inappropriate formats (e.g., “<”, “>”, or zero where not applicable) may not be considered.
Read and follow the PT Protocol carefully before starting analysis.

PT Result Submission

EL-F-01-7.3-05

Program Code: 2026MF44R1

Last Date for Result Submission: 05th August 2026

Note: All fields are mandatory.

Before starting the analysis, please ensure you have read the handling and analysis instructions carefully.

Lab Code(Required)

Please enter a number from 0 to 20.

For lab code, refer to the sample forwarding letter sent along with the sample

Name of Participating Laboratory/ Organization:(Required)

As filled in the registration form

Date of Result Reporting(Required)

Sample Code(Required)

For sample code, refer to the sample forwarding letter sent along with the sample

Name(Required)

Designation(Required)

Phone(Required)

Email(Required)

Start Date of Analysis(Required)

End Date of Analysis(Required)

Section 1

Sample Vial(Required)

Please select the appropriate vial code

Measurand/Parameter(Required)

Please select the appropriate measurand

Participating ?(Required)

Results(Required)

Units of Measurement(Required)

Method Used(Required)

Media Used(Required)

Colony characteristics(Required)

Incubation Conditions(Required)

e.g. 37 Degrees for 24 hrs

Biochemical Performed(Required)

Any Confirmatory/Biochemical Tests Performed and their results

Accredited as per ISO 17025 scope?(Required)

Section 2

Sample Vial(Required)

Please select the appropriate vial code

Measurand/Parameter(Required)

Please select the appropriate measurand

Participating ?(Required)

Results(Required)

Units of Measurement(Required)

Method Used(Required)

Media Used(Required)

Colony characteristics(Required)

Incubation Conditions(Required)

e.g. 37 Degrees for 24 hrs

Biochemical Test(Required)

Any Confirmatory/Biochemical Tests Performed and their results

Accredited as per ISO 17025 scope?(Required)

Section 3

Sample Vial(Required)

Please select the appropriate vial code

Measurand/Parameter(Required)

Please select the appropriate measurand

Participating ?(Required)

Results(Required)

Units of Measurement(Required)

Method Used(Required)

Media Used(Required)

Colony Charateristics(Required)

Incubation Conditions(Required)

e.g. 37 Degrees for 24 hrs

Biochemical Test(Required)

Any Confirmatory/Biochemical Tests Performed and their results

Accredited as per ISO 17025 scope?(Required)

Section 4

Sample Vial(Required)

Please select the appropriate vial code

Measurand/Parameter(Required)

Please select the appropriate measurand

Participating ?(Required)

Results(Required)

Please enter a number greater than or equal to 1.

Do not enter values in decimal points or symbols “greater than” or “less than”, etc. Values reported less that 2 or 1.8 wont be considered for final evaluation as per the standards, these values will be marked as RNC in the final report.

Units of Measurement(Required)

Method Used(Required)

Media Used(Required)

Colony Charateristics(Required)

Incubation Conditions(Required)

e.g. 37 Degrees for 24 hrs

Accredited as per ISO 17025 scope?(Required)

Section 5

Sample Vial(Required)

Please select the appropriate vial code

Measurand/Parameter(Required)

Please select the appropriate measurand

Participating ?(Required)

Results(Required)

Please enter a number greater than or equal to 1.

Units of Measurement(Required)

Method Used(Required)

Media Used(Required)

Colony Charateristics(Required)

Incubation Conditions(Required)

e.g. 37 Degrees for 24 hrs

Accredited as per ISO 17025 scope?(Required)

2026MF44R1 Instruction sheet and Result submission

PT Round 2026MF44R1

Instructions to Participants

Quick Safety & Handling

Program Details

Revival Instructions

Vial 2026MF44R1-V1

Vial 2026MFF44R1-V2

Vial 2026MFF44R1-V3

Vial 2026MFF44R1-V4

Raw Data Submission

Reporting of Results

Analytes to Report

Key Instructions (Read before you begin)

PT Result Submission

Section 1

Section 2

Section 3

Section 4

Section 5

Organizations’ Details

Registered Office

Quick link

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