PT Round 2025MF06R2 Step 1 of 5 20% Instructions to Participants Please read carefully before starting analysis Quick Safety & Handling Only trained and authorized personnel should handle PT items. On receiving the PT item/sample, check that it is intact. If there is any issue, inform the PTP immediately. Record this in the sample receipt acknowledgement link shared by email and submit it within two days of receipt. Record date of receipt and store under the specified conditions until analysis. Initiate analysis within 48 hours of receipt. Treat PT items like routine samples and use standard methods unless otherwise instructed. Read the detailed instructions/information and follow them accordingly. Open PT containers only when you begin the analysis. Program Details Program Code: 2025MF06R2 Category: Milk and Dairy Products Measurand: E. coli, Salmonella and L. monocytogenes Result Submission: 05 Feb 2026 Storage: 2–8 °C until analysis Revival Instructions for Vials Vial 2025MF06R2-V1 Uncap the sample vial 2025MF06R2-V1 aseptically and rehydrate using 4ml sterile saline peptone water (0.1% peptone with 0.85% NaCl). Securely recap and allow 5 minutes rehydration at room temperature. Aseptically upcap container 2025MF06R2-1 and transfer 25g of the sample matrix to the sterile enrichment broth for Salmonella and remaining 25g to the sterile enrichment broth for L. monocytogenes. Homogenize thoroughly Transfer 2ml rehydrated vial to Salmonella enrichment broth and transfer remaining 2ml to L. monocytogenes enrichment broth. Perform routine Analysis for Salmonella and L. monocytogenes Vial 2025MF06R2-V2 Uncap the sample vial 2025MF06R2-V2 aseptically and rehydrate using 4ml sterile saline peptone water (0.1% peptone with 0.85% NaCl). Securely recap and allow 5 minutes rehydration at room temperature. Aseptically upcap container 2025MF06R2-2 and transfer 25g of the sample matrix to the E. coli diluting fluid. Homogenize thoroughly. Empty rehydrated vial to E. coli diluting fluid . Perform routine Analysis for E. coli Raw Data Submission Clear photographs of all plates (before counting, with readable labelling). Photographs of all biochemical / confirmation tests performed (if applicable). Accepted formats: PDF | Max size: 5 MB each. Ensure photos are clear, in focus, and labelled with sample code, date and dilution (if used). Note: Ensure images are in focus and labeled with sample ID, dilution, and date. Reporting of Results PT Round: 2025MF06R2 Analytes to Report For this PT round 2025MF06R2, you are required to report results for the following analytes: Salmonella Reporting Basis: per 25g • IS 5887 (Part 3): 1999• ISO 6579 (Part 1): 2017 Listeria monocytogenes Reporting Basis: 25g • BAM chapter 10• IS 14988 (Part 1): 2020 E.coli Reporting Basis: per 25g • IS 5887 (Part 1): 1976 Online submission only: Do not download/print this page. Use the Next button to proceed to result entry. Key Instructions (Read before you begin) Do NOT download or print this page…results must be entered and submitted online only. Use the Next button at the bottom to go to the results entry section. Double-check all entries before submission — changes may not be accepted after Final submission. Retain all raw data and supporting records (e.g., plate photographs, biochemical results, calibration logs) as per ISO/IEC 17025. Upload clear evidence where required — unclear/missing records may lead to disqualification. Avoid collusion — responsibility lies entirely with the participant. Click Submit once only — unsolicited re-submissions may result in only the first entry being considered. If you cannot proceed, ensure all required fields are completed. You will see a confirmation message upon successful submission. Use the specified methods, or an equivalent validated method capable of required accuracy. Obtain prior written permission for deviations. Results in inappropriate formats (e.g., “less than”, “More than”, or zero where not applicable) may not be considered. Read and follow the PT Protocol carefully before starting analysis. Consent(Required) I have read, understood, and agree to the above instructions and conditions.Please click on the consent to proceed with the result submission. PT Result Submission Program Code: 2025MF06R2 Last Date for Result Submission: 05th February 2026 Note: All fields are mandatory. Before starting the analysis, please ensure you have read the handling and analysis instructions carefully. Lab Code(Required)Please enter a number from 0 to 15.For lab code, refer to the sample forwarding letter sent along with the sampleName of Participating Laboratory/ Organization:(Required) Date of Result Reporting(Required) DD dot MM dot YYYY As filled in the registration formName(Required) Designation(Required) Phone(Required)Email(Required) Start Date of Analysis(Required) DD dot MM dot YYYY End Date of Analysis(Required) DD dot MM dot YYYY Section 1Sample Vial(Required)2025MF06R2-12025MF06R2-2Please select the appropriate vial codeMeasurand/Parameter(Required)SalmonellaL.monocytogenesE.coliPlease select the appropriate measurandParticipating ?(Required)Select from the DropdownYes – I will submit results for this parameterNo – I am not participating in this parameterResult(Required)DetectedNot detectedPresentAbsentUnits of Measurement(Required)per 25gmper gMethod Used(Required)Any other equivalent methodIS 5887 (Part 3):1999ISO 6579 (Part 1):2017BAM chapter 10IS 14988 (Part 1):2020IS 5887 (Part 1):1976Media Used(Required)Incubation Conditions(Required)e.g. 37 Degrees for 24 hrsBiochemical Performed(Required)Any Confirmatory/Biochemical Tests Performed and their resultsAccredited as per ISO 17025 scope?(Required)YesNoApplicantSection 2Sample Vial(Required)2025MF06R2-12025MF06R2-2Please select the appropriate vial codeMeasurand/Parameter(Required)L. monocytogenesSalmonellaE. coliPlease select the appropriate measurandParticipating ?(Required)Select from the DropdownYes – I will submit results for this parameterNo – I am not participating in this parameterResults(Required)DetectedNot DetectedPresentAbsentUnits of Measurement(Required)per 25gper gMethod Used(Required)Any other equivalent methodIS 5887 (Part 3):1999ISO 6579 (Part 1):2017BAM chapter 10IS 14988 (Part 1):2020IS 5887 (Part 1):1976Media Used(Required)Incubation Conditions(Required)e.g. 37 Degrees for 24 hrsBiochemical Test(Required)Any Confirmatory/Biochemical Tests Performed and their resultsAccredited as per ISO 17025 scope?(Required)YesNoApplicantSection 3Sample Vial(Required)2025MF06R2-22025MF06R2-1Please select the appropriate vial codeMeasurand/Parameter(Required)E. coliL. monocytogenesSalmonellaPlease select the appropriate measurandParticipating ?(Required)Select from the DropdownYes – I will submit results for this parameterNo – I am not participating in this parameterResults(Required)DetectedNot DetectedPresentAbsentUnits of Measurement(Required)per 25gper gMethod Used(Required)Any other equivalent methodIS 5887 (Part 3):1999ISO 6579 (Part 1):2017BAM chapter 10IS 14988 (Part 1):2020IS 5887 (Part 1):1976Media Used(Required)Incubation Conditions(Required)e.g. 37 Degrees for 24 hrsBiochemical Test(Required)Any Confirmatory/Biochemical Tests Performed and their resultsUpload Raw Data(Required)Max. file size: 512 MB.Upload clear photographs of plates, positive tubes and biochemical tests performed for all measurand or parameter tested. Equipment Used(Required)Reference Material/s Used (Include Traceability))(Required)Were all the equipment used calibrated ?(Required)Choose from Drop DownYesNo(Choose Yes/No as per the applicability) Organizations’ DetailsAccredited I Mention the discipline(Required)BiologicalChemicalApplicantApplicant I Mention the discipline If NABL accredited mention TC No(Required) e.g. TC xxxxxTested By(Required) Designation(Required) Authorized By(Required) Designation(Required) Consent(Required) I confirm that all results are accurate and obtained as per scheme instructions.I hereby confirm that I am authorized to submit this form. I have thoroughly checked and updated all required fields to ensure accuracy. I fully understand that once this form is submitted, the results cannot be altered under any circumstances. Furthermore, I acknowledge and accept full responsibility for the accuracy and completeness of all information provided in this submission.Section Break
