Instructions and Result Submission 2025MF04R1

PT Round 2025MF04R1

Step 1 of 5

Instructions to Participants

Only trained and authorized personnel should handle PT items.
Confirm sample integrity upon receipt and notify the PT Provider if damaged.
Record date of receipt and store under the specified conditions until analysis.
Initiate analysis within 48 hours of receipt.
Treat PT items like routine samples and use standard methods unless otherwise instructed.

Program Details

Program Code: 2025MF04R1 | Fruits and Vegetable Products

Matrix: Deydrated Vegetable Powder

Measurand: Total Plate Count, Enterobacteriaceae, S.aureus and Yeast & Mould

Last Date of Result Submission: 20th September 2025

Storage Condition: 2–8°C until analysis

Revival Instructions

Vial 2025MF04R1-V1

Aseptically uncap the vial and rehydrate with 5 ml sterile saline peptone water (0.1% Peptone + 0.85% NaCl).
Securely recap and allow 5 minutes rehydration at room temperature.
Uncap container 2025MF04R1-1:
- Transfer 10 g of sample matrix to sterile bag/bottle/flask.
- Empty the vial 2025MF04R1-V1 to 85ml of dilluent ensuring thorough homogenization of the sample.
- Perform serial dillution at (10¹ – 10⁵) and proceed with your routine analysis of total plate count and Enteroabacteriaceae.

Vial 2025MF04R1-V2

Aseptically uncap the vial and rehydrate with 5 ml sterile saline peptone water (0.1% Peptone + 0.85% NaCl).
Securely recap and allow 5 minutes rehydration at room temperature.
Uncap container 2025MF04R1-1:
- Aseptically transfer 10 g of sample matrix to sterile bag/bottle/flask.
- Empty the vial 2025MF04R1-V2 to 85ml of diluent ensuring thorough homogenization.
- Perform serial dillution at (10¹ – 10⁵) and proceed with your routine analysis of Yeast and Mould.

Vial 2025MF04R1-V3

Aseptically uncap the vial and rehydrate with 5 ml sterile saline peptone water (0.1% Peptone + 0.85% NaCl).
Securely recap and allow 5 minutes rehydration at room temperature.
Uncap container 2025MF04R1-1:
- Aseptically transfer 10 g of sample matrix to sterile bag/bottle/flask.
- Empty the vial 2025MF04R1-V2 to 85ml of diluent ensuring thorough homogenization.
- Perform serial dillution at (10¹ – 10⁵) and proceed with your routine analysis of S. aureus .

Raw Data Submission Requirements

Upload clear photographs of plates for all analytes tested.
Upload photographs of biochemical tests performed.
Accepted formats: JPG, PNG, PDF | Max size: 5 MB each.

Reporting of Results

For this PT round 2025MF04R1, you are required to report results for the following analytes:

Total Plate Count — cfu/g | IS 5402 (Part 1):2021 / BAM chapter 3
Enterobacteriaceae — cfu/g | ISO 21528 (Part 2):2017
Yeast & Mould — cfu/g | IS 5403:1999 / BAM chapter 18
S. aureus — cfu/g | IS 5887 (Part 2):1976/ BAM chapter 12

Key Instructions (Read before you begin)

Do NOT download or print this page — results must be entered and submitted online only.
Use the Next button at the bottom to go to the results entry section.
Double-check all entries before submission — changes may not be accepted after submission.
Retain all raw data and supporting records (e.g., photos of plates, biochemical test results, calibration logs) as per ISO/IEC 17025 requirements.
Upload clear evidence where required — unclear or missing records may lead to disqualification.
Do not engage in collusion — responsibility lies entirely with the participant.
Click the Submit button once only — re-submissions without request may result in only the first entry being considered.
If you cannot proceed, check that all required fields are completed.
You will see a confirmation message once your results are successfully submitted.
Use the specified methods, or an equivalent validated method capable of achieving required accuracy. Obtain prior written permission for deviations.
Results in inappropriate formats (e.g., “less than”, “more than”, or zero where not applicable) may not be considered.
Read and follow the PT Protocol carefully before starting analysis.

PT Result Submission

Program Code: 2025MF04R1

Last Date for Result Submission: 20th September 2025

Note: All fields are mandatory.

Before starting the analysis, please ensure you have read the handling and analysis instructions carefully.

Lab Code(Required)

Please enter a number from 0 to 17.

For lab code, refer to the sample forwarding letter sent along with the sample

Name of Participating Laboratory/ Organization:(Required)

Date of Result Reporting(Required)

As filled in the registration form

Name(Required)

Designation(Required)

Phone(Required)

Email(Required)

Start Date of Analysis(Required)

End Date of Analysis(Required)

Section 1

Sample Vial(Required)

Please select the appropriate vial code

Measurand/Parameter(Required)

Please select the appropriate measurand

Participating ?(Required)

Results(Required)

Enter your results as whole number only. Do not type decimals, present or absent. Only required format is accepted.

Units of Measurement(Required)

Method Used(Required)

Media Used(Required)

Dilutions plated(Required)

Incubation Conditions(Required)

e.g. 37 Degrees for 24 hrs

Biochemical Performed(Required)

Any Confirmatory/Biochemical Tests Performed and their results

Accredited as per ISO 17025 scope?(Required)

Section 2

Sample Vial(Required)

Please select the appropriate vial code

Measurand/Parameter(Required)

Please select the appropriate measurand

Participating ?(Required)

Results(Required)

Enter your results as whole number only. Do not type decimals, present or absent. Only required format is accepted.

Units of Measurement(Required)

Method Used(Required)

Media Used(Required)

Dilution Plated(Required)

Incubation Conditions(Required)

e.g. 37 Degrees for 24 hrs

Biochemical Test(Required)

Any Confirmatory/Biochemical Tests Performed and their results

Accredited as per ISO 17025 scope?(Required)

Section 3

Sample Vial(Required)

Please select the appropriate vial code

Measurand/Parameter(Required)

Please select the appropriate measurand

Participating ?(Required)

Results(Required)

Enter your results as whole number only. Do not type decimals, present or absent. Only required format is accepted.

Units of Measurement(Required)

Method Used(Required)

Media Used(Required)

Dilution Plated(Required)

Incubation Conditions(Required)

e.g. 37 Degrees for 24 hrs

Biochemical Test(Required)

Any Confirmatory/Biochemical Tests Performed and their results

Accredited as per ISO 17025 scope?(Required)

Section 4

Sample Vial(Required)

Please select the appropriate vial code

Measurand/Parameter(Required)

Please select the appropriate measurand

Participating ?(Required)

Results

Enter your results as whole number only. Do not type decimals, present or absent. Only required format is accepted.

Units of Measurement(Required)

Method Used(Required)

Media Used(Required)

Colony Charateristics(Required)

Incubation Conditions(Required)

e.g. 37 Degrees for 24 hrs

Biochemical Test(Required)

Any Confirmatory/Biochemical Tests Performed and their results

Accredited as per ISO 17025 scope?(Required)

Upload Raw Data(Required)

Max. file size: 512 MB.

Upload clear photographs of plates, positive tubes and biochemical tests performed for all measurand or parameter tested.

Instructions and Result Submission 2025MF04R1

PT Round 2025MF04R1

Instructions to Participants

Program Details

Revival Instructions

Vial 2025MF04R1-V1

Vial 2025MF04R1-V2

Vial 2025MF04R1-V3

Raw Data Submission Requirements

Reporting of Results

Key Instructions (Read before you begin)

PT Result Submission

Section 1

Section 2

Section 3

Section 4

Organizations’ Details

Registered Office

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