Instructions to Participants:
- Only trained and authorized personnel should handle PT items.
- Confirm sample integrity upon receipt and notify the PT Provider if damaged.
- Record date of receipt and store under the specified conditions until analysis.
- Initiate analysis within 48 hours of receipt.
- Treat PT items like routine samples and use standard methods unless otherwise instructed.
Program Code: 2025MF06R1|Milk and Dairy Products
Matrix: Milk Powder
Measurand: E. coli, Salmonella, L. monocytogenes
Last date of result submission: 25th August 2025
Store till Analysis: 2–8°C
Instructions to revive the sample vial 2025MF06R1-V1:
- Aseptically uncap the vial and rehydrate with 4 ml sterile saline peptone water. (0.1% Peptone and 0.85% NaCl)
- Securely recap and allow 5 minutes rehydration at room temperature.
- Aseptically upcap container 2025MF06R1-1 and transfer 25g of the sample matrix to the sterile enrichment broth for Salmonella and remaining 25g to the dilluting fluide prepared for E.coli.
Homogenize this. Transfer 2ml rehydrated vial to Salmonella enrichment broth and transfer remaining 2ml to E.coli dilluting fluids.
- Perform routine Analysis for:
Instructions to revive the sample vial 2025MF06R1-V2
- Aseptically uncap the vial and rehydrate with 4 ml sterile saline peptone water. (0.1% Peptone and 0.85% NaCl)
- Securely recap and allow 5 minutes rehydration at room temperature.
- Aseptically upcap container 2025MF06R1-2 and transfer 25g of the sample matrix to the sterile enrichment broth for L. monocytogenes.
Homogenize this. Empty rehydrated vial to
L. monocytogenes enrichment broth.
- Perform routine Analysis for:
Raw Data Submission Requirements:
- Upload clear photographs of plates for all analytes tested.
- Upload photographs of biochemical tests performed.
- Accepted formats: JPG, PNG, PDF | Max size: 5MB each.