INSTRUCTIONS FOR HANDLING AND ANALYSIS
A. Receipt, Handling & Storage
Precaution: It is expected that participating laboratories ensure PT items are handled by trained and authorized personnel who possess the necessary competence and awareness regarding the specific handling procedures, safety precautions related to the test parameters, and the hazards associated with the chemicals involved. Should any uncertainty arise during the handling of PT items, please contact the designated personnel at +91-9167702413 or via email at pt@envirocare.co.in for clarification.
Please note below points prior to initiate the analysis:
- Upon receipt of the PT item/sample, confirm if the sample is intact & immediately inform the PTP if needed.
- Record the date of receiving and store properly till the analysis is performed.
- Initiate the analysis within 48 hours of receiving the sample.
- The test materials should be treated in the same way as routine samples and should therefore be tested using your routine laboratory equipment, reagents, and methods.
- The entire content of the vials & container must be utilized for analysis.
- Read all the instructions carefully before analysis.
Note: Participants are expected to use the specified method(s) mentioned below (Reporting of Results) or any other validated methods based on these specified methods.
| PT Round | 2025MF05R1 |
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| Matrix | Coriander Powder |
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| Analyte | Total Plate Count, Salmonella and Bacillus cereus
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| PT item details |
- 1 Sterile Plastic Container with approx. 50 g PT item matrix coriander powder product marked: 2025MF05R1-1.
- 1 Sterile Plastic Container with approx. 15 g PT item matrix coriander powder product marked: 2025MF05R1-2.
- 2 Lyophilized vials in sterile plastic containers marked: 2025MF05R1-V1, 2025MF05R1-V2.
- 1 Lyophilized vials in sterile plastic containers marked: 2025MF05R1-V3.
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| Last date for result submission: | 30.05.2025 |
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B. Instructions for Analysis: Specific
For: 2025MF05R1-V1
- Uncap the sample vials 2025MF05R1-V1 aseptically and rehydrate using 4ml sterile saline peptone water (0.1% peptone with 0.85% NaCl).
- Recap securely & allow 5 minutes at room temperature for rehydration.
- Aseptically transfer 25 g of the PT item matrix from 2025MF05R1-1 to the sterile enrichment broth for Salmonella. Homogenize this.
- Empty the rehydrate vial content into the diluent prepared in step 3, ensuring thorough homogenization of the sample.
- proceed with your routine analysis for Salmonella.
For: 2025MF05R1-V2
- Uncap the sample vials 2025MF05R1-V2 aseptically and rehydrate using 5ml sterile saline peptone water (0.1% peptone with 0.85% NaCl).
- Recap securely & allow 5 minutes at room temperature for rehydration.
- Aseptically transfer the remaining 25 g of the sample from 2025MF05R1-1 to the sterile enrichment broth for Salmonella and Homogenize this.
- Empty the rehydrate vial content into the diluent prepared in step 3, ensuring thorough homogenization of the sample.
- Proceed with your routine analysis for Salmonella.
For: 2025MF05R1-V3
- Uncap the sample vials 2025MF05R1-V3 aseptically and rehydrate using 5ml sterile saline peptone water (0.1% peptone with 0.85% NaCl).
- Recap securely & allow 5 minutes at room temperature for rehydration.
- Aseptically transfer 10 g of the sample from 2025MF05R1-2 to the sterile bag/bottle/flask & add 85 ml of diluent to it.
- Empty the rehydrate vial content into the diluent prepared in step 3, ensuring thorough homogenization of the sample.
- Perform serial dilution (101 – 105) & proceed with your routine analysis for Total Plate Count and B.cereus.
