PT Round 2026MF45R1 Step 1 of 6 16% Instructions to Participants EL-F-01-7.3-06 Please read carefully before starting analysis Quick Safety & Handling Only trained and authorized personnel should handle PT items. Confirm sample integrity upon receipt; notify the PT Provider if damaged. Record date of receipt and store under the specified conditions until analysis. Initiate analysis within 48 hours of receipt. Treat PT items like routine samples and use standard methods unless otherwise instructed. Program Details Program Code: 2026MF45R1 Category: Meat and Meat Products Matrix: Meat powder Measurand: Escherichia coli 0157, and Campylobacter spp. Result Submission: 31 August 2026 Storage: 2–8 °C until analysis Revival Instructions Vial 2026MF45R1-V1 Uncap the sample vial 2026MF45R1-V1 aseptically and rehydrate using 4 ml sterile saline peptone water (0.1% peptone with 0.85% NaCl). Recap securely & allow 5 minutes at room temperature for rehydration. Aseptically transfer 25 g of the sample matrix from 2026MF45R1 to the sterile enrichment broth for Escherichia coli 0157 and transfer the remaining 10 g of the sample matrix to the special diluting fluid for Campylobacter spp. Homogenize this and transfer 2 ml of each of the rehydrated vial content from 2026MF45R1-V1 to the enrichment broth for Escherichia coli 0157 and Campylobacter spp. Proceed with your routine analysis. Raw Data Submission Upload clear photographs of plates for all analytes tested. Upload photographs of biochemical tests performed. Accepted formats: JPG, PNG, PDF | Max size: 5 MB each. Note: Ensure images are in focus and labeled with sample ID, dilution, and date. Reporting of Results PT Round: 2026MF45R1 Analytes to Report For this PT round 2026MF45R1, you are required to report results for the following analytes: Escherichia coli 0157 Reporting Basis: per 25g ISO 16654:2001 Campylobacter spp. Reporting Basis: per 10g IS0 10272 (Part 1):2017 Online submission only: Do not download/print this page. Use the Next button to proceed to result entry. Key Instructions (Read before you begin) Do NOT download or print this page — results must be entered and submitted online only. Use the Next button at the bottom to go to the results entry section. Double-check all entries before submission — changes may not be accepted after submission. Retain all raw data and supporting records (e.g., plate photographs, biochemical results, calibration logs) as per ISO/IEC 17025. Upload clear evidence where required — unclear/missing records may lead to disqualification. Avoid collusion — responsibility lies entirely with the participant. Click Submit once only — unsolicited re-submissions may result in only the first entry being considered. If you cannot proceed, ensure all required fields are completed. You will see a confirmation message upon successful submission. Use the specified methods, or an equivalent validated method capable of required accuracy. Obtain prior written permission for deviations. Results in inappropriate formats (e.g., “<”, “>”, or zero where not applicable) may not be considered. Read and follow the PT Protocol carefully before starting analysis. Consent(Required) I have read, understood, and agree to the above instructions and conditions.Please click on the consent to proceed with the result submission. PT Result Submission EL-F-01-7.3-05 Program Code: 2026MF45R1 Last Date for Result Submission: 31st August 2026 Note: All fields are mandatory. Before starting the analysis, please ensure you have read the handling and analysis instructions carefully. Lab Code(Required)Please enter a number from 0 to 20.For lab code, refer to the sample forwarding letter sent along with the sampleName of Participating Laboratory/ Organization:(Required) As filled in the registration formDate of Result Reporting(Required) DD dot MM dot YYYY Name(Required) Designation(Required) Phone(Required)Email(Required) Start Date of Analysis(Required) DD dot MM dot YYYY End Date of Analysis(Required) DD dot MM dot YYYY Section 1Sample Vial(Required)2026MF45R1-V1Please select the appropriate vial codeMeasurand/Parameter(Required)Escherichia coli 0157Please select the appropriate measurandParticipating ?(Required)Yes – I will submit results for this parameterNo – I am not participating in this parameterResults(Required)DetectedNot DetectedPresentAbsentUnits of Measurement(Required)per 25gMethod Used(Required)Any other equivalent methodISO 16654:2001Media Used(Required)Colony characteristics(Required)Incubation Conditions(Required)e.g. 37 Degrees for 24 hrsBiochemical Performed(Required)Any Confirmatory/Biochemical Tests Performed and their resultsAccredited as per ISO 17025 scope?(Required)YesNoApplicantSection 2Sample Vial(Required)2026MF45R1-V1Please select the appropriate vial codeMeasurand/Parameter(Required)Campylobacter spp.Please select the appropriate measurandParticipating ?(Required)Yes – I will submit results for this parameterNo – I am not participating in this parameterResults(Required)DetectedNot DetectedPresentAbsentUnits of Measurement(Required)per 10gMethod Used(Required)Any other equivalent methodIS0 10272 (Part 1):2017Media Used(Required)Colony characteristics(Required)Incubation Conditions(Required)e.g. 37 Degrees for 24 hrsBiochemical Test(Required)Any Confirmatory/Biochemical Tests Performed and their resultsAccredited as per ISO 17025 scope?(Required)YesNoApplicant Upload Raw Data(Required)Max. file size: 512 MB.Upload clear photographs of plates, positive tubes and biochemical tests performed for all measurand or parameter tested. Equipment Used(Required)Reference Material/s Used (Include Traceability))(Required)Were all the equipment used calibrated ?(Required)Choose from Drop DownYesNo(Choose Yes/No as per the applicability) Organizations’ DetailsAccredited I Mention the discipline(Required)BiologicalChemicalApplicantApplicant I Mention the discipline If NABL accredited mention TC No(Required) e.g. TC xxxxxTested By(Required) Designation(Required) Authorized By(Required) Designation(Required) Consent(Required) I confirm that all results are accurate and obtained as per scheme instructions.I hereby confirm that I am authorized to submit this form. I have thoroughly checked and updated all required fields to ensure accuracy. I fully understand that once this form is submitted, the results cannot be altered under any circumstances. Furthermore, I acknowledge and accept full responsibility for the accuracy and completeness of all information provided in this submission.