PT Round 2025MF25R1 Step 1 of 5 20% Instructions to Participants Please read carefully before starting analysis Quick Safety & Handling Only trained and authorized personnel should handle PT items. Confirm sample integrity upon receipt; notify the PT Provider if damaged. Record date of receipt and store under the specified conditions until analysis. Initiate analysis within 48 hours of receipt. Treat PT items like routine samples and use standard methods unless otherwise instructed. Program Details Program Code: 2025MF25R1 Category: Animal Food and Feed Matrix: Soybean meal Measurand: Salmonella, Escherichia coli Result Submission: 10 Dec 2025 Storage: 2–8 °C until analysis Revival Instructions Vial 2025MF25R1-V1 Uncap the sample vial 2025MF25R1-V1 aseptically and rehydrate using 4ml sterile saline peptone water (0.1% peptone with 0.85% NaCl). Recap securely & allow 5 minutes at room temperature for rehydration. Aseptically transfer 25 g of the sample matrix from the container 2025MF25R1-1 to enrichment broth for Salmonella and the remaining 25g into diluting fluid for E. coli by ensuring proper homogenization. Transfer 2ml from vial 2025MF25R1-V1 to the Salmonella enrichment broth and remaining 2ml into E.coli diluting fluid ensuring proper homogenization. Proceed with your routine analysis for Salmonella and Escherichia coli. Revival Instructions Vial 2025MF25R1-V2 Uncap the sample vial 2025MF25R1-V2 aseptically and rehydrate using 4ml sterile saline peptone water (0.1% peptone with 0.85% NaCl). Recap securely & allow 5 minutes at room temperature for rehydration. Aseptically transfer 25 g of the sample matrix from the container 2025MF25R1-2 to enrichment broth for Salmonella and the remaining 25g into diluting fluid for E. coli by ensuring proper homogenization. Transfer 2ml from vial 2025MF25R1-V2 to the Salmonella enrichment broth and remaining 2ml into E.coli diluting fluid ensuring proper homogenization. Proceed with your routine analysis for Salmonella and Escherichia coli. Raw Data Submission Upload clear photographs of plates for all analytes tested. Upload photographs of biochemical tests performed. Accepted formats: JPG, PNG, PDF. Note: Ensure images are in focus and labeled with sample ID, dilution, and date. Reporting of Results PT Round: 2025MF25R1 Analytes to Report For this PT round 2025MF25R1, you are required to report results for the following analytes: Salmonella Reporting Basis: 25g IS 5887 (Part 3);1999, BAM chapter 5 Escherichia coli Reporting Basis: Per 25g IS 5887 (Part 1);1976 Online submission only: Do not download/print this page. Use the Next button to proceed to result entry. Key Instructions (Read before you begin) Do NOT download or print this page — results must be entered and submitted online only. Use the Next button at the bottom to go to the results entry section. Double-check all entries before submission — changes may not be accepted after submission. Retain all raw data and supporting records (e.g., plate photographs, biochemical results, calibration logs) as per ISO/IEC 17025. Upload clear evidence where required — unclear/missing records may lead to disqualification. Avoid collusion — responsibility lies entirely with the participant. Click Submit once only — unsolicited re-submissions may result in only the first entry being considered. If you cannot proceed, ensure all required fields are completed. You will see a confirmation message upon successful submission. Use the specified methods, or an equivalent validated method capable of required accuracy. Obtain prior written permission for deviations. Results in inappropriate formats (e.g., “<”, “>”, or zero where not applicable) may not be considered. Read and follow the PT Protocol carefully before starting analysis. Consent(Required) I have read, understood, and agree to the above instructions and conditions.Please click on the consent to proceed with the result submission. PT Result Submission Program Code: 2025MF25R1 Last Date for Result Submission: 10th December 2025 Note: All fields are mandatory. Before starting the analysis, please ensure you have read the handling and analysis instructions carefully. Lab Code(Required)Please enter a number from 0 to 50.For lab code, refer to the sample forwarding letter sent along with the sampleName of Participating Laboratory/ Organization:(Required) Date of Result Reporting(Required) DD dot MM dot YYYY As filled in the registration formName(Required) Designation(Required) Phone(Required)Email(Required) Start Date of Analysis(Required) DD dot MM dot YYYY End Date of Analysis(Required) DD dot MM dot YYYY Section 1Sample Vial(Required)2025MF25R1-V12025MF25R1-V2Please select the appropriate vial codeMeasurand/Parameter(Required)L. monocytogenesS. aureusSalmonellaE.coliPlease select the appropriate measurandParticipating ?(Required)Yes – I will submit results for this parameterNo – I am not participating in this parameterResults(Required)AbsentPresentDetectedNot DetectedEnter your results as “Present”, “Absent” only. Do not type numbers, “<”, “>” or “0”. Only required format is accepted.Units of Measurement(Required)Per 100mlper 25gMethod Used(Required)IS 5887 (Part 1):1976Any other equivalent methodIS 5887 (Part 3):1999ISO 6579 (Part 1):2017BAM chapter 5Media Used(Required)Colony Characteristics(Required)Incubation Conditions(Required)e.g. 37 Degrees for 24 hrsBiochemical Performed(Required)Any Confirmatory/Biochemical Tests Performed and their resultsAccredited as per ISO 17025 scope?(Required)YesNoApplicantSection 2Sample Vial(Required)2025MF25R1-V12025MF25R1-V2Please select the appropriate vial codeMeasurand/Parameter(Required)SalmonellaE. coliL. monocytogenesS. aureusPlease select the appropriate measurandParticipating ?(Required)Yes – I will submit results for this parameterNo – I am not participating in this parameterResults(Required)AbsentPresentDetectedNot DetectedEnter your results as “Present”, “Absent” only. Do not type numbers, “<”, “>” or “0”. Only required format is accepted.Units of Measurement(Required)Per 25gPer 10gMethod Used(Required)IS 5887 (Part 1):1976Any other equivalent methodIS 5887 (Part 3):1999BAM chapter 5IS 15185:2016Media Used(Required)Colony Charateristics(Required)Incubation Conditions(Required)e.g. 37 Degrees for 24 hrsBiochemical Test(Required)Any Confirmatory/Biochemical Tests Performed and their resultsAccredited as per ISO 17025 scope?(Required)YesNoApplicantSection 3Sample Vial(Required)2025MF25R1-V12025MF25R1-V2Please select the appropriate vial codeMeasurand/Parameter(Required)SalmonellaE. coliS. aureusShigellaPlease select the appropriate measurandParticipating ?(Required)Yes – I will submit results for this parameterNo – I am not participating in this parameterResults(Required)AbsentPresentDetectedNot DetectedEnter your results as “Present”, “Absent” only. Do not type numbers, “<”, “>” or “0”. Only required format is accepted.Units of Measurement(Required)Per gPer 25gMethod Used(Required)IS 5887 (Part 1):1976Any other equivalent methodIS 5887 (Part 3):1999IS 5887 (Part 2):1976BAM chapter 5IS 1622:1981Media Used(Required)Colony Charateristics(Required)Incubation Conditions(Required)e.g. 37 Degrees for 24 hrsBiochemical Test(Required)Any Confirmatory/Biochemical Tests Performed and their resultsAccredited as per ISO 17025 scope?(Required)YesNoApplicantSection 4Sample Vial(Required)2025MF25R1-V12025MF25R1-V2Please select the appropriate vial codeMeasurand/Parameter(Required)SalmonellaE. coliL. monocytogenesP. aeruginosaPlease select the appropriate measurandParticipating ?(Required)Yes – I will submit results for this parameterNo – I am not participating in this parameterResults(Required)AbsentPresentDetectedNot DetectedEnter your results as “Present”, “Absent” only. Do not type numbers, “<”, “>” or “0”. Only required format is accepted.Units of Measurement(Required)Per gPer 25gPer 100mlMethod Used(Required)IS 5887 (Part 1):1976Any other equivalent methodISO 6579 (Part 1):2017IS 5887 (Part 2):1976BAM chapter 5IS 5887 (Part 3):1999IS 15185:2016Media Used(Required)Colony Charateristics(Required)Incubation Conditions(Required)e.g. 37 Degrees for 24 hrsBiochemical Test(Required)Any Confirmatory/Biochemical Tests Performed and their resultsAccredited as per ISO 17025 scope?(Required)YesNoApplicantUpload Raw Data(Required)Max. file size: 512 MB.Upload clear photographs of plates, positive tubes and biochemical tests performed for all measurand or parameter tested. Equipment Used(Required)Reference Material/s Used (Include Traceability))(Required)Were all the equipment used calibrated ?(Required)Choose from Drop DownYesNo(Choose Yes/No as per the applicability) Organizations’ DetailsAccredited I Mention the discipline(Required)BiologicalChemicalApplicantApplicant I Mention the discipline If NABL accredited mention TC No(Required) e.g. TC xxxxxTested By Designation(Required) Authorized By Designation(Required) Consent(Required) I confirm that all results are accurate and obtained as per scheme instructions.I hereby confirm that I am authorized to submit this form. I have thoroughly checked and updated all required fields to ensure accuracy. I fully understand that once this form is submitted, the results cannot be altered under any circumstances. Furthermore, I acknowledge and accept full responsibility for the accuracy and completeness of all information provided in this submission.
