PT Round 2025MB01R1 Step 1 of 6 16% Instructions to Participants Please read carefully before starting analysis Quick Safety & Handling Only trained and authorized personnel should handle PT items. Confirm sample integrity upon receipt; notify the PT Provider if damaged. Record date of receipt and store under the specified conditions until analysis. Initiate analysis within 48 hours of receipt. Treat PT items like routine samples and use standard methods unless otherwise instructed. Program Details Program Code: 2025MB01R1 Category: Biocides Matrix: Sanitizer Measurand: Escherichia coli and Staphylococcus aureus Result Submission: 05 Jan 2026 Storage: 2–8 °C until analysis 2025MB01R1 Test procedure for antimicrobial efficacy Perform Antimicrobial efficacy test with the original sample provided as PT sample marked 2025MB01R1. Take 9.0 ml PT sample + 1 ml inoculum of test organisms like E. coli and S. aureus. Take these standard cultures with initial count at zero time as > 1 x 106 cfu/ml. Keep the exposure time /contact time 60 Seconds. Perform the plate count of E. coli and S. aureus as per your routine procedure at exposure time 60 Seconds, by taking 1 ml aliquot from test suspension and transferring to 9 ml neutralizer/diluent. Perform serial dilution as required and determine colony count after the exposure time. Calculations Determine log value of organism count (cfu /ml) for initial count and after the exposure time. Calculate the Log10 reduction (LR) & % reduction using the below formula. Log10 reduction (LR) compared to control at Zero Time = (Initial Log value – Final Log value). Percent reduction compared to control at zero Time = [100 × (1 – 10-LR)] Reporting of results Report the results as antimicrobial efficacy as Log reduction and Percent reduction (1) E. coli (2) S. aureus. Samples should be treated like any other samples and all normal quality control procedures should be adopted. Raw Data Submission Upload clear photographs of plates for all analytes tested. Upload photographs of biochemical tests performed. Accepted formats: JPG, PNG, PDF. Note: Ensure images are in focus and labeled with sample ID, dilution, and date. Reporting of Results PT Round: 2025MB01R1 Analytes to Report For this PT round 2025MB01R1, you are required to report results for the following analytes: Escherichia coli Reporting Basis: Per 25 ml ASTM E2315 Staphylococcus aureus Reporting Basis: Per 25 ml ASTM E2315 Online submission only: Do not download or print this page. Use the Next button to proceed to result entry. Key Instructions (Read before you begin) Do NOT download or print this page — results must be entered online only. Use the Next button at the bottom to go to the results entry section. Double-check all entries before submission. Retain all raw data and supporting records as per ISO/IEC 17025. Upload clear evidence where required. Avoid collusion — responsibility lies entirely with the participant. Click Submit once only. Ensure all required fields are completed. You will see a confirmation message upon successful submission. Use specified or validated equivalent methods only. Inappropriate result formats may not be considered. Read and follow the PT Protocol carefully. Consent(Required) I have read, understood, and agree to the above instructions and conditions.Please click on the consent to proceed with the result submission. PT Result Submission Program Code: 2025MB01R1 Last Date for Result Submission: 05th January 2025 Note: All fields are mandatory. Before starting the analysis, please ensure you have read the handling and analysis instructions carefully. Lab Code(Required)Please enter a number from 0 to 20.For lab code, refer to the sample forwarding letter sent along with the sampleName of Participating Laboratory/ Organization:(Required) Date of Result Reporting(Required) DD dot MM dot YYYY As filled in the registration formName(Required) Designation(Required) Phone(Required)Email(Required) Start Date of Analysis(Required) DD dot MM dot YYYY End Date of Analysis(Required) DD dot MM dot YYYY Section 1Sample Vial(Required)2025MB01R1Please select the appropriate vial codeMeasurand/Parameter(Required)Escherichia coliStaphylococcus aureusPlease select the appropriate measurandParticipating ?(Required)Yes – I will submit results for this parameterNo – I am not participating in this parameterContact Time(Required)Zero Timecfu/ml(Required) Log10 value(Required)Log10 reduction (X.XX)(Required)Contact Time(Required)60 Secondscfu/ml(Required) Log10 value(Required)Log10 reduction (X.XX)(Required)% Reduction (XX.XX)(Required)Method Used(Required)Any other equivalent methodASTM E2315Incubation Conditions(Required)e.g. 37 Degrees for 24 hrsAccredited as per ISO 17025 scope?(Required)YesNoApplicantSection 2Sample Vial(Required)2025MB01R1Please select the appropriate vial codeMeasurand/Parameter(Required)Escherichia coliStaphylococcus aureusPlease select the appropriate measurandParticipating ?(Required)Yes – I will submit results for this parameterNo – I am not participating in this parameterContact Time(Required)Zero Timecfu/ml(Required) Log10 value(Required)Log10 reduction (X.XX)(Required)Contact Time(Required)60 Secondscfu/ml(Required) Log10 value(Required)Log10 reduction (X.XX)(Required)% Reduction (XX.XX)(Required)Method Used(Required)Any other equivalent methodUSEPA 1602ASTM E2315Incubation Conditions(Required)e.g. 37 Degrees for 24 hrsAccredited as per ISO 17025 scope?(Required)YesNoApplicant Upload Raw Data(Required)Max. file size: 512 MB.Upload clear photographs of plates, positive tubes and biochemical tests performed for all measurand or parameter tested. Equipment Used(Required)Reference Material/s Used (Include Traceability))(Required)Were all the equipment used calibrated ?(Required)Choose from Drop DownYesNo(Choose Yes/No as per the applicability) Organizations’ DetailsAccredited I Mention the discipline(Required)BiologicalChemicalApplicantApplicant I Mention the discipline If NABL accredited mention TC No(Required) e.g. TC xxxxxTested By(Required) Designation(Required) Authorized By(Required) Designation(Required) Consent(Required) I confirm that all results are accurate and obtained as per scheme instructions.I hereby confirm that I am authorized to submit this form. I have thoroughly checked and updated all required fields to ensure accuracy. I fully understand that once this form is submitted, the results cannot be altered under any circumstances. Furthermore, I acknowledge and accept full responsibility for the accuracy and completeness of all information provided in this submission.UntitledFirst ChoiceSecond ChoiceThird ChoiceUntitled
